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I haven't read anything that would hint that he's so consumed with sex that he wouldn't care about her mental state. We haven't seen them do anything but have sex and maybe this passion is what their relationship is built on instead of something more solid which would give her comfort in this time of uncertainty. I would avoid her like the plague as well even if I was happy about it. But to be really honest, we've only seen them sexually. I hope this didn't come across argumentative as I asked out of curiosity. Actually, I am very happy that you were Right and I was Wrong.I based that prediction on the accusation delivered to Dan by his boss along with his facial expression.I have mixed feelings about her and her situation, to pity her or think she deserves it for being irresponsible.But anyway great visual storytelling as always, thank you for sharing your works with us.One N-glycosylation site is present in Xyn D amino acid sequence at position N101, suggesting that N-glycosylation is responsible for the higher molecular mass.To confirm this change was due to glycosylation, deglycosylation analysis was performed.I took that as Dan has a long history of hitting on the co-eds who come to him for help/ that some may still be ongoing/ that none presumably led to an engagement or marriage or worse that they did.I had no problem with there being an age difference or that he has some authority over her as long as the university had no policy against this type of relationship--hypocrite that I am.

The purified recombinant enzyme migrated in SDS-PAGE give a single band around 64 k Da (Figure 2, lane 2).Obviously there were off-stage interactions and discussions going on we are not privy to.Oh, and I sensed nary a smidgen of argumentation or hostility in your comments.After treatment with Endo H to remove N-linked carbohydrate moieties, only a single band located at about 60 k Da was observed (Figure 2, lane 3) which confirms that Xyn D was N-glycosylated.However, the molecular mass was still higher than that expected and periodic acid Schiff staining still positive, suggesting the presence of O-glycosylated moieties (Figure 2, lane 7).




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